# Platform Biologist, CRISPR Screening

Live page: https://affinitytalent.bio/jobs/platform-biologist-crispr-screening

## Role summary

- Series A functional genomics company
- Cambridge, MA, US
- On-site · Mid-level
- Compensation: $132k - $158k + equity + bonus

Run pooled CRISPR screens that identify disease-relevant targets in oncology and immune-biology models.

## About the role

The company runs a functional-genomics platform used to find and validate therapeutic targets across oncology and immune-biology discovery programs. As a Platform Biologist, you will run pooled CRISPR screens from cell culture and viral transduction through selection, gDNA preparation, QC, and sequencing handoff. The team needs a careful bench scientist who can keep coverage, controls, and documentation strong as screen volume grows.

## Responsibilities

- Screen execution: Run pooled CRISPR workflows including cell expansion, lentiviral transduction, antibiotic or marker selection, sampling, and genomic DNA preparation.
- Quality control: Monitor MOI, guide representation, replicate consistency, cell health, selection pressure, and positive/negative controls so weak screens are caught early.
- Process documentation: Maintain clear protocols, batch records, coverage calculations, culture notes, sequencing-prep records, and troubleshooting history.
- Biology and data handoff: Work with computational biology and assay teams to review QC, explain screen limitations, and prioritize follow-up validation assays.

## Requirements

- MS with 3+ years, or BS with 5+ years, in pooled CRISPR screening, functional genomics, perturbation screening, or related molecular biology workflows.
- Hands-on experience with mammalian tissue culture, lentiviral transduction or infection, selection conditions, and BSL-2 practices.
- Working understanding of MOI, guide coverage, representation, replicate quality, positive and negative controls, and dropout behavior.
- Comfort preparing genomic DNA or sequencing-ready material and partnering with computational colleagues on screen readouts.
- Strong experimental judgment around artifacts, batch effects, cell-health issues, and when a screen should be repeated or redesigned.

## Technical stack

Pooled CRISPR screening, lentiviral transduction, mammalian tissue culture, MOI/coverage tracking, BSL-2 workflows, genomic DNA prep, FACS/flow cytometry, NGS QC, MAGeCK/CRISPResso.